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CD14 antibody [LPSR/2386]

Anti-CD14 antibody [LPSR/2386] used in Flow cytometry (FACS). GTX17873
Anti-CD14 antibody [LPSR/2386] used in Western Blot (WB). GTX17873
Anti-CD14 antibody [LPSR/2386] used in IHC (Paraffin sections) (IHC-P). GTX17873
Anti-CD14 antibody [LPSR/2386] used in IHC (Paraffin sections) (IHC-P). GTX17873
Anti-CD14 antibody [LPSR/2386] used in Protein Array (Protein Array). GTX17873

Cat. No. GTX17873

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LPSR/2386

Isotype

 IgG1

Application

 WB, IHC-P, FACS, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 , CD14 molecule

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody [LPSR/2386] used in Flow cytometry (FACS). GTX17873

GTX17873 FACS Image

FACS analysis of A549 cells using GTX17873 CD14 antibody [LPSR/2386].
Blue : Primary antibody
Red : Isotype control

Anti-CD14 antibody [LPSR/2386] used in Western Blot (WB). GTX17873

GTX17873 WB Image

WB analysis of A549 cell lysate using GTX17873 CD14 antibody [LPSR/2386].

Anti-CD14 antibody [LPSR/2386] used in IHC (Paraffin sections) (IHC-P). GTX17873

GTX17873 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX17873 CD14 antibody [LPSR/2386].

Anti-CD14 antibody [LPSR/2386] used in IHC (Paraffin sections) (IHC-P). GTX17873

GTX17873 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX17873 CD14 antibody [LPSR/2386].

Anti-CD14 antibody [LPSR/2386] used in Protein Array (Protein Array). GTX17873

GTX17873 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD14 Mouse Monoclonal Antibody (LPSR/2386). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529