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CD14 antibody [MEM-18] (FITC)

Anti-CD14 antibody [MEM-18] (FITC) used in Flow cytometry (FACS). GTX75931

Cat No. GTX75931

Host

Mouse

Clonality

Monoclonal

Clone Name

MEM-18

Isotype

IgG1

Application

FACS

Reactivity

Human, Primate
Package
100 test ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

PROPERTIES

Form

Liquid

Buffer

PBS, 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Purification

Purified by size-exclusion chromatography

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD14 , CD14 molecule

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody [MEM-18] (FITC) used in Flow cytometry (FACS). GTX75931

GTX75931 FACS Image

FACS analysis of human peripheral blood leukocytes using GTX75931 CD14 antibody [MEM-18] (FITC).

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf