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United States (US)

CD14 antibody [MEM-18]

CD14-antibody-MEM-18__GTX28103-1_18121409_620.gif
CD14-antibody-MEM-18__GTX28103-2_18121409_993.gif
CD14-antibody-MEM-18__GTX28103-1_18121409_943.jpg
GTX28103_CD14-antibody-MEM-18__GTX28103-1_19012723_769.jpg

Cat No. GTX28103

Host Mouse
Clonality Monoclonal
Clone Name MEM-18
Isotype IgG1
Application WB, FACS, IP, ELISA, Functional Assay
Reactivity Human, Monkey, Primate
APPLICATION

Application Note

ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. Blocks binding of bacterial LPS to the monocyte surface. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

CD14 antigen (human)
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Purification

Purified by sequential precipitation (caprylic acid and ammonium sulphate) followed by DEAE-cellulose chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cytoplasmic. Attached to the membrane by a GPI anchor.

Background

CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram-negative shock.

Database

Research Area

DATA IMAGES
CD14-antibody-MEM-18__GTX28103-1_18121409_620.gif

GTX28103 FACS Image

Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC (GTX75931)

CD14-antibody-MEM-18__GTX28103-2_18121409_993.gif

GTX28103 FACS Image

Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE (GTX75932)

CD14-antibody-MEM-18__GTX28103-1_18121409_943.jpg

GTX28103 WB Image

Western Blot analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18) (GTX28103).
Lane 1: whole cell lysate HEK 293 transfected with empty vector
Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA
Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA

GTX28103_CD14-antibody-MEM-18__GTX28103-1_19012723_769.jpg

GTX28103 FACS Image

Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) FITC (GTX75931).

REFERENCE
Package List Price ($)
$ 319