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CD14 antibody [MEM-18]

Anti-CD14 antibody [MEM-18] used in Western Blot (WB). GTX28103

Cat. No. GTX28103

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-18

Isotype

 IgG1

Application

 WB, FACS, IP, ELISA, Sandwich ELISA

Reactivity

 Human, Primate
Package
100 μg ($399)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX28103, Detection : GTX42422
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_369136

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 , CD14 molecule

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody [MEM-18] used in Western Blot (WB). GTX28103

GTX28103 WB Image

WB analysis of various samples (non-reducing) using GTX28103 CD14 antibody [MEM-18].
Lane 1 : HEK 293 transfected with empty vector
Lane 2 : tissue culture supernatant of HEK 293 transfected with human CD14 cDNA
Lane 3 : HEK 293 transfected with human CD14

REFERENCE

Application Reference

Lowry MB et al. J Steroid Biochem Mol Biol 2014; 143 : 183-91 Regulation of the human cathelicidin antimicrobial peptide gene by 1α,25-dihydroxyvitamin D3 in primary immune cells.
Application : FACS
Reactivity : Human

Clone Reference

Su LJ et al. Sci Rep 2017; 7:45607 Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399