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CD14 antibody [TÜK4] (Low endotoxin, azide free)

Anti-CD14 antibody [TÜK4] (Low endotoxin, azide free) used in ELISA (ELISA). GTX42422

Cat. No. GTX42422

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TÜK4

Isotype

 IgG2a

Application

 WB, FACS, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

 Human, Rabbit, Goat, Sheep, Bovine, Cat, Dog, Pig, Cynomolgus monkey, Llama, Mink
Package
100 μg ($399)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS 1/100-1/200
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Neutralizing/Inhibition
Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003).

Sandwich ELISA
Capture : GTX28103, Detection : GTX42422
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody recognizes the human CD14 cell surface antigen.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein A purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 molecule

Cellular Localization

Cell membrane , Secreted , Golgi apparatus

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody [T&#220;K4] (Low endotoxin, azide free) used in ELISA (ELISA). GTX42422

GTX42422 ELISA Image

Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (GTX28103) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tuk4(GTX42422) as a detection reagent. Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively

REFERENCE

Clone Reference

Liao XY et al. Medicine (Baltimore) 2017; 96 (39):e8143 Intraoperative blood salvage may shorten the lifespan of red blood cells within 3 days postoperatively: A pilot study.
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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations