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CD146 antibody [6C3E6]

Anti-CD146 antibody [6C3E6] used in Western Blot (WB). GTX60775
Anti-CD146 antibody [6C3E6] used in IHC (Paraffin sections) (IHC-P). GTX60775
Anti-CD146 antibody [6C3E6] used in Flow cytometry (FACS). GTX60775
Anti-CD146 antibody [6C3E6] used in IHC (Paraffin sections) (IHC-P). GTX60775
Anti-CD146 antibody [6C3E6] used in Western Blot (WB). GTX60775
Anti-CD146 antibody [6C3E6] used in ELISA (ELISA). GTX60775

Cat. No. GTX60775

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C3E6

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all CD146 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD146 (AA: 84-189) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2888001

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanoma cell adhesion molecule , CD146 , HEMCAM , METCAM , MUC18 , MelCAM

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD146 antibody [6C3E6] used in Western Blot (WB). GTX60775

GTX60775 WB Image

WB analysis of HEK293 (1) and CD146 (AA: 84-189)-hIgGFc transfected HEK293 (2) cell lysate using GTX60775 CD146 antibody [6C3E6].

Anti-CD146 antibody [6C3E6] used in IHC (Paraffin sections) (IHC-P). GTX60775

GTX60775 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60775 CD146 antibody [6C3E6].

Anti-CD146 antibody [6C3E6] used in Flow cytometry (FACS). GTX60775

GTX60775 FACS Image

FACS analysis of MCF-7 cells using GTX60775 CD146 antibody [6C3E6].
Green : CD146
Red : negative control

Anti-CD146 antibody [6C3E6] used in IHC (Paraffin sections) (IHC-P). GTX60775

GTX60775 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60775 CD146 antibody [6C3E6].

Anti-CD146 antibody [6C3E6] used in Western Blot (WB). GTX60775

GTX60775 WB Image

WB analysis of HUVE-12 (1), EVC-304 (2), HeLa (3) and MCF-7 (4) cell lysate using GTX60775 CD146 antibody [6C3E6].

Anti-CD146 antibody [6C3E6] used in ELISA (ELISA). GTX60775

GTX60775 ELISA Image

ELISA analysis of antigen using GTX60775 CD146 antibody [6C3E6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

Application Reference

Zhao C et al. Oncol Rep 2020; 44 (2) : 674-684 Supervillin promotes tumor angiogenesis in liver cancer.
Heidemann F et al. PLoS One 2014; 9 (4) : e92327 Selectins mediate small cell lung cancer systemic metastasis.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409