GeneTex
United States (US)

CD146 antibody [C146/634]

Anti-CD146 antibody [C146/634] used in IHC (Paraffin sections) (IHC-P). GTX34460
Anti-CD146 antibody [C146/634] used in IHC (Paraffin sections) (IHC-P). GTX34460

Cat No. GTX34460

Host Mouse
Clonality Monoclonal
Clone Name C146/634
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1.0ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Specificity/Sensitivity

The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human CD146 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-CD146 antibody [C146/634] used in IHC (Paraffin sections) (IHC-P). GTX34460

GTX34460 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD146 Monoclonal Antibody (C146/634)

Anti-CD146 antibody [C146/634] used in IHC (Paraffin sections) (IHC-P). GTX34460

GTX34460 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with CD146 Monoclonal Antibody (C146/634)

Package List Price ($)
$ 329