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CD146 antibody [C1C3]

KO/KD Orthogonal
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

Cat. No. GTX108777

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD146 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

rat liver , HUVEC , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729041

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanoma cell adhesion molecule , CD146 , HEMCAM , METCAM , MUC18 , MelCAM

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Database

Research Area

DATA IMAGES

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

KO/KD

Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD146 stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

CD146 antibody [C1C3] detects CD146 protein by Western blot analysis.
A. 30 μg HUVEC whole cell lysate/extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:10000

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD146 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108777

GTX108777 ICC/IF Image

CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

CD146 antibody [C1C3] detects CD146 protein by western blot analysis.
A. 50 μg rat liver extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:1000

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD146 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108777

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD146 antibody [C1C3] used in Western Blot (WB). GTX108777

GTX108777 WB Image

Orthogonal

The data was published in the journal Stem Cells Transl Med in 2020.PMID: 31943851

REFERENCE

Application Reference

Suardi D et al. Med Sci Monit 2023; 29 : e939125 Comparison of Extravillous Intermediate Trophoblast Invasion Depth and Distribution Pattern Between Placenta Accreta and Non-Accreta.
Application : IHC-P
Reactivity : Human
Hu Y et al. J Orthop Translat 2020; 24 : 12-22 Defactinib attenuates osteoarthritis by inhibiting positive feedback loop between H-type vessels and MSCs in subchondral bone.
Pearse DD et al. Neuroscience 2020; (Epub) Neuronal and Endothelial Transglutaminase-2 Expression during Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis.
Application : IHC-P
Reactivity : Rat
Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Akiyama Y et al. Proc Natl Acad Sci U S A 2019; 116 (16) : 7738-7743 Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169