GeneTex
United States (US)

CD146 antibody [C1C3]

GTX108777_41122_20181109_WB_KO_watermark_19011519_387.jpg
GTX108777_40401_IHC_19011507_846.jpg
GTX108777_41122_IHC_M_19011507_541.jpg
GTX108777_41122_20161027_IFA_19011507_891.jpg
GTX108777_40401_WB_R_liver_19011507_933.jpg
GTX108777_41122_WB_19011507_956.jpg
GTX108777_41122_IHC_M_2_19011507_509.jpg

Cat No. GTX108777

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

HUVEC , rat liver extract
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Melanoma Cell Adhesion Molecule,Cd146,Hemcam,Metcam,Muc18,Melcam,Mcam

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Database

Research Area

DATA IMAGES
GTX108777_41122_20181109_WB_KO_watermark_19011519_387.jpg

GTX108777 WB Image

Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX108777_40401_IHC_19011507_846.jpg

GTX108777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108777_41122_IHC_M_19011507_541.jpg

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108777_41122_20161027_IFA_19011507_891.jpg

GTX108777 ICC/IF Image

CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX108777_40401_WB_R_liver_19011507_933.jpg

GTX108777 WB Image

CD146 antibody [C1C3] detects CD146 protein by western blot analysis.
A. 50 μg rat liver extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:1000

GTX108777_41122_WB_19011507_956.jpg

GTX108777 WB Image

CD146 antibody [C1C3] detects CD146 protein by Western blot analysis.
A. 30 μg HUVEC whole cell lysate/extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:10000

GTX108777_41122_IHC_M_2_19011507_509.jpg

GTX108777 IHC-P Image

CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169