CD146 antibody [HL6385]

Wild-type (WT) and MET knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD146 antibody [HL6385] (GTX648824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

CD146 antibody [HL6385] detects CD146 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
CD146 stained by CD146 antibody [HL6385] (GTX648824) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD146 antibody [HL6385] detects CD146 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CD146 stained by CD146 antibody [HL6385] (GTX648824) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD146 antibody [HL6385] detects CD146 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
CD146 stained by CD146 antibody [HL6385] (GTX648824) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD146 antibody [HL6385] (GTX648824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Boiled and unboiled whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD146 antibody [HL6385] (GTX648824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD146 antibody [HL6385] (GTX648824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.