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CD146 antibody [N1N3]

KO/KD
Anti-CD146 antibody [N1N3] used in Western Blot (WB). GTX102413
Anti-CD146 antibody [N1N3] used in Western Blot (WB). GTX102413
Anti-CD146 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102413

Cat. No. GTX102413

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD146 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A375 , HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD146. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617509

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanoma cell adhesion molecule , CD146 , HEMCAM , METCAM , MUC18 , MelCAM

Background

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Database

Research Area

DATA IMAGES

Anti-CD146 antibody [N1N3] used in Western Blot (WB). GTX102413

GTX102413 WB Image

KO/KD

Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [N1N3] (GTX102413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD146 antibody [N1N3] used in Western Blot (WB). GTX102413

GTX102413 WB Image

CD146 antibody detects CD146 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with CD146 antibody (GTX102413) diluted by 1:1000

Anti-CD146 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX102413

GTX102413 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CD146(GTX102413) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169