GeneTex
United States (US)

CD146 antibody [N1N3]

KO/KD
GTX102413_40275_20181109_WB_KO_watermark_19011519_256.jpg
GTX102413_40275_IHC_19011506_579.jpg
GTX102413_40275_WB_19011506_277.jpg

Cat No. GTX102413

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

A375

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CD146. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD146 antibody, MUC18 antibody, MCAM antibody, melanoma adhesion molecule antibody, Gicerin antibody, melanoma-associated antigen A32 antibody, cell surface glycoprotein MUC18 antibody, cell surface glycoprotein P1H12 antibody, S-endo 1 endothelial-associated antigen antibody, melanoma-associated antigen MUC18 antibody, melanoma cell adhesion molecule antibody

Background

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Database

Research Area

DATA IMAGES
GTX102413_40275_20181109_WB_KO_watermark_19011519_256.jpg

GTX102413 WB Image

KO/KD

Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [N1N3] (GTX102413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102413_40275_IHC_19011506_579.jpg

GTX102413 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CD146(GTX102413) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102413_40275_WB_19011506_277.jpg

GTX102413 WB Image

CD146 antibody detects CD146 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with CD146 antibody (GTX102413) diluted by 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169