CD147 antibody [4E4]

Name: CD147 antibody [4E4]
Brand: GeneTex
SKU: GTX84798
Price: 519.00 USD

Anti-CD147 antibody [4E4] used in Flow cytometry (FACS). GTX84798

Anti-CD147 antibody [4E4] used in IHC (Paraffin sections) (IHC-P). GTX84798

Anti-CD147 antibody [4E4] used in Western Blot (WB). GTX84798

Anti-CD147 antibody [4E4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84798

Cat. No. GTX84798

Host	Mouse
Clonality	Monoclonal
Clone Name	4E4
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($519)

See all CD147 products

Datasheet
Document

View product citations for antibody GTX84798 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basigin (Ok blood group) , 5F7 , CD147 , EMMPRIN , EMPRIN , OK , SLC7A11 , TCSF

Cellular Localization

Cell membrane

Background

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

Database

Gene ID: 682 BSG
UniProt: P35613 BSG

Research Area

DATA IMAGES

GTX84798 FACS Image

FACS analysis of Jurkat cells using GTX84798 CD147 antibody [4E4].
Red : Primary antibody
Blue : Negative control antibody

GTX84798 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84798 CD147 antibody [4E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84798 IHC-P Image

IHC-P analysis of human colon tissue using GTX84798 CD147 antibody [4E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84798 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84798 CD147 antibody [4E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84798 WB Image

WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84798 CD147 antibody [4E4].
Loading : 5 ug per lane

GTX84798 IHC-P Image

IHC-P analysis of human lung tissue using GTX84798 CD147 antibody [4E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84798 WB Image

WB analysis of wild type and CD147 knock out 293T cell lysate(10μg per lane) using GTX84798 CD147 antibody [4E4].
Dilution : 1:2000

GTX84798 WB Image

WB analysis of HeLa cell lysate using GTX84798 CD147 antibody [4E4].
Loading : 10 ug per lane
Dilution : 1:200

GTX84798 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84798 CD147 antibody [4E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84798 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84798 CD147 antibody [4E4].

GTX84798 IHC-P Image

IHC-P analysis of human liver tissue using GTX84798 CD147 antibody [4E4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

CD147 antibody [4E4]

Cat. No. GTX84798

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84798 FACS Image

GTX84798 IHC-P Image

GTX84798 IHC-P Image

GTX84798 IHC-P Image

GTX84798 WB Image

GTX84798 IHC-P Image

GTX84798 WB Image

GTX84798 WB Image

GTX84798 IHC-P Image

GTX84798 ICC/IF Image

GTX84798 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.