CD147 antibody [5D8]

Name: CD147 antibody [5D8]
Brand: GeneTex
SKU: GTX84795
Price: 519.00 USD

Anti-CD147 antibody [5D8] used in IHC (Paraffin sections) (IHC-P). GTX84795

Anti-CD147 antibody [5D8] used in Western Blot (WB). GTX84795

Anti-CD147 antibody [5D8] used in Flow cytometry (FACS). GTX84795

Anti-CD147 antibody [5D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84795

Cat. No. GTX84795

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	5D8
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($519)

See all CD147 products

View product citations for antibody GTX84795 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10729303

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basigin (Ok blood group) , 5F7 , CD147 , EMMPRIN , EMPRIN , OK , SLC7A11 , TCSF

Cellular Localization

Cell membrane

Background

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

Database

Gene ID: 682 BSG
UniProt: P35613 BSG

Research Area

DATA IMAGES

GTX84795 IHC-P Image

IHC-P analysis of human liver tissue using GTX84795 CD147 antibody [5D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84795 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84795 CD147 antibody [5D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84795 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84795 CD147 antibody [5D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84795 WB Image

WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84795 CD147 antibody [5D8].
Loading : 5 ug per lane

GTX84795 FACS Image

FACS analysis of HEK293T cells transfected with either CD147 plasmid(Red) or empty vector control plasmid(Blue) using GTX84795 CD147 antibody [5D8].

GTX84795 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84795 CD147 antibody [5D8].

GTX84795 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84795 CD147 antibody [5D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84795 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84795 CD147 antibody [5D8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84795 FACS Image

FACS analysis of Jurkat cells using GTX84795 CD147 antibody [5D8].
Red : Primary antibody
Blue : Negative control antibody

GTX84795 FACS Image

FACS analysis of HeLa cells using GTX84795 CD147 antibody [5D8].
Red : Primary antibody
Blue : Negative control antibody

GTX84795 WB Image

WB analysis of HeLa cell lysate using GTX84795 CD147 antibody [5D8].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

Application Reference

Walters DK et al. Cell Cycle 2013; 12 (19) : 3175-83 CD147 regulates the expression of MCT1 and lactate export in multiple myeloma cells.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW

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Application Reference

Walters DK et al. Cell Cycle 2013; 12 (19):3175-83 CD147 regulates the expression of MCT1 and lactate export in multiple myeloma cells.

Application : WB

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

CD147 antibody [5D8]

Cat. No. GTX84795

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84795 IHC-P Image

GTX84795 IHC-P Image

GTX84795 IHC-P Image

GTX84795 WB Image

GTX84795 FACS Image

GTX84795 ICC/IF Image

GTX84795 IHC-P Image

GTX84795 IHC-P Image

GTX84795 FACS Image

GTX84795 FACS Image

GTX84795 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.