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CD147 antibody [8D6]

Anti-CD147 antibody [8D6] used in IHC (Paraffin sections) (IHC-P). GTX34462

Cat. No. GTX34462

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8D6

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($529)
See all CD147 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:200
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Neutralizing/Inhibition Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.


Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migation through Matrigel. For functional assay, please contact us to order PBS only format.
Not tested in other applications.

Calculated MW

0|42 kDa. ( Note )

Product Note

This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human T cell line Molt 13

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BSG , CD147 , EMMPRIN , OK , TCSF , basigin (Ok blood group) , 5F7

Cellular Localization

Cell membrane,Melanosome

Background

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD147 antibody [8D6] used in IHC (Paraffin sections) (IHC-P). GTX34462

GTX34462 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX34462 CD147 antibody [8D6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529