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CD147 antibody [9H5]

KO/KD Overexpression
Anti-CD147 antibody [9H5] used in IHC (Paraffin sections) (IHC-P). GTX84801
Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801
Anti-CD147 antibody [9H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84801
Anti-CD147 antibody [9H5] used in Flow cytometry (FACS). GTX84801
Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801
Anti-CD147 antibody [9H5] used in IHC (Paraffin sections) (IHC-P). GTX84801
Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801
Anti-CD147 antibody [9H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84801
Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801

Cat. No. GTX84801

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9H5

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($489)
See all CD147 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basigin (Ok blood group) , 5F7 , CD147 , EMMPRIN , EMPRIN , OK , SLC7A11 , TCSF

Cellular Localization

Cell membrane

Background

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

Database

Research Area

DATA IMAGES

Anti-CD147 antibody [9H5] used in IHC (Paraffin sections) (IHC-P). GTX84801

GTX84801 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84801 CD147 antibody [9H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801

GTX84801 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84801 CD147 antibody [9H5].
Loading : 5 ug per lane

Anti-CD147 antibody [9H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84801

GTX84801 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84801 CD147 antibody [9H5].

Anti-CD147 antibody [9H5] used in Flow cytometry (FACS). GTX84801

GTX84801 FACS Image

FACS analysis of HEK293T cells transfected with either CD147 plasmid(Red) or empty vector control plasmid(Blue) using GTX84801 CD147 antibody [9H5].

Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801

GTX84801 WB Image

KO/KD

WB analysis of wild type and CD147 knock out 293T cell lysate(10μg per lane) using GTX84801 CD147 antibody [9H5].
Dilution : 1:2000

Anti-CD147 antibody [9H5] used in IHC (Paraffin sections) (IHC-P). GTX84801

GTX84801 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84801 CD147 antibody [9H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801

GTX84801 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84801 CD147 antibody [9H5].
Loading : 10 ug per lane
Dilution : 1:500

Anti-CD147 antibody [9H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84801

GTX84801 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84801 CD147 antibody [9H5].

Anti-CD147 antibody [9H5] used in Western Blot (WB). GTX84801

GTX84801 WB Image

WB analysis of various cell lines using GTX84801 CD147 antibody [9H5].
Loading : 35 ug per lane

REFERENCE

Application Reference

Takenaga K et al. Sci Rep 2021; 11 (1) : 13302 MCT4 is induced by metastasis-enhancing pathogenic mitochondrial NADH dehydrogenase gene mutations and can be a therapeutic target.
Application : WB
Reactivity : Mouse
Emon ST et al. World Neurosurg 2021; 149 : e982-e988 Effects of Ozone on Injury after Gamma Knife Radiosurgery.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489