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CD147 antibody [MEM-M6/1] (Azide free)

Anti-CD147 antibody [MEM-M6/1] (Azide free) used in Western Blot (WB). GTX75485
Anti-CD147 antibody [MEM-M6/1] (Azide free) used in Flow cytometry (FACS). GTX75485

Cat. No. GTX75485

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-M6/1

Isotype

 IgG1

Application

 WB, FACS, IP

Reactivity

 Human
Package
500 μg ($489)
See all CD147 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS 1/10-1/50
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domai

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.

Purification

Purified by hydrophobic charge induction chromatography

Conjugation

Unconjugated

RRID

AB_376835

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basigin (Ok blood group) , 5F7 , CD147 , EMMPRIN , EMPRIN , OK , SLC7A11 , TCSF

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD147 antibody [MEM-M6/1] (Azide free) used in Western Blot (WB). GTX75485

GTX75485 WB Image

WB analysis of HepG2 cell lysate using GTX75485 CD147 antibody [MEM-M6/1] (Azide free).

Anti-CD147 antibody [MEM-M6/1] (Azide free) used in Flow cytometry (FACS). GTX75485

GTX75485 FACS Image

FACS analysis of human erythrocytes using GTX75485 CD147 antibody [MEM-M6/1] (Azide free).

REFERENCE

Clone Reference

Nikas IP et al. Cancer Med 2022; Biomarkers expression among paired serous ovarian cancer primary lesions and their peritoneal cavity metastases in treatment-na?ve patients: A single-center study.
Kornek M et al. Hepatology 2011; 53 (1):230-42 Human T cell microparticles circulate in blood of hepatitis patients and induce fibrolytic activation of hepatic stellate cells.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 489
Custom Conjugations