APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Observed MW
36 kDa. The observed M.W. is based on the following publication. PMID: 21641389
Positive Control
K562 membrane extract
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CD147. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
basigin (Ok blood group),5F7,CD147,EMMPRIN,EMPRIN,OK,SLC7A11,TCSF
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Melanosome , Cell membrane , Melanosome
Background
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915](/upload/website/prouct_img/middle/GTX100915/GTX100915_39897_IHC_19011506_512.jpg "Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915")
|
GTX100915 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using CD147(GTX100915) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
![Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915](/upload/website/prouct_img/middle/GTX100915/GTX100915_39897_20160114_WB_Fraction_19011506_748.jpg "Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915")
|
GTX100915 WB Image
K562 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD147 antibody [N2C3] (GTX100915) diluted at 1:500.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications. Reference: PMID: 21641389
|
REFERENCE
There are currently no references for CD147 antibody [N2C3] (GTX100915). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD147 antibody [N2C3] (GTX100915). Be the first to share your experience with this product.
![Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915](/upload/website/prouct_img/small/GTX100915/GTX100915_39897_IHC_19011506_512.jpg "Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915")
![Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915](/upload/website/prouct_img/small/GTX100915/GTX100915_39897_20160114_WB_Fraction_19011506_748.jpg "Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915")
![Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915](/upload/website/prouct_img/normal/GTX100915/GTX100915_39897_IHC_19011506_512.jpg "Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915")
![Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915](/upload/website/prouct_img/normal/GTX100915/GTX100915_39897_20160114_WB_Fraction_19011506_748.jpg "Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915")
