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CD147 antibody [N2C3]

Orthogonal
Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915
Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915

Cat No. GTX100915

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all CD147 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Observed MW

36 kDa. The observed M.W. is based on the following publication. PMID: 21641389

Positive Control

K562 membrane extract

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD147. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

basigin (Ok blood group) , 5F7 , CD147 , EMMPRIN , EMPRIN , OK , SLC7A11 , TCSF

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Melanosome , Cell membrane , Melanosome

Background

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD147 antibody [N2C3] used in Western Blot (WB). GTX100915

GTX100915 WB Image

Orthogonal

K562 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD147 antibody [N2C3] (GTX100915) diluted at 1:500.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications. Reference: PMID: 21641389

Anti-CD147 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100915

GTX100915 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using CD147(GTX100915) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169