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CD151 antibody [11G5a]

Anti-CD151 antibody [11G5a] used in Flow cytometry (FACS). GTX42394

Cat. No. GTX42394

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11G5a

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human
Package
25 μg ($169)
See all CD151 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/100
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent
ELISA Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD151 molecule (Raph blood group) , GP27 , MER2 , PETA-3 , RAPH , SFA1 , TSPAN24

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD151 antibody [11G5a] used in Flow cytometry (FACS). GTX42394

GTX42394 FACS Image

FACS analysis of human peripheral blood platelets using GTX42394 CD151 antibody [11G5a].

REFERENCE

Clone Reference

Yang XH et al. J Cell Sci 2012; 125 (Pt 6):1478-87 CD151 restricts the α6 integrin diffusion mode.
Yang XH et al. Cancer Res 2008; 68 (9):3204-13 CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization.
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169