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CD155 antibody

Anti-CD155 antibody used in Western Blot (WB). GTX129572
Anti-CD155 antibody used in Western Blot (WB). GTX129572

Cat No. GTX129572

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , K562 , THP-1 , HL-60

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD155. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poliovirus receptor , CD155 , HVED , NECL5 , Necl-5 , PVS , TAGE4

Cellular Localization

Isoform Alpha: Cell membrane; Single-pass type I membrane protein , Isoform Delta: Cell membrane; Single-pass type I membrane protein , Isoform Beta: Secreted , Isoform Gamma: Secreted

Background

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD155 antibody used in Western Blot (WB). GTX129572

GTX129572 WB Image

Wild-type (WT) and CD155 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD155 antibody (GTX129572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD155 antibody used in Western Blot (WB). GTX129572

GTX129572 WB Image

CD155 antibody detects CD155 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
C. 30 μg HL-60 whole cell lysate/extract
10 % SDS-PAGE
CD155 antibody (GTX129572) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169