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CD16 + CD32 antibody [2.4G2] (Biotin)

Anti-CD16 + CD32 antibody [2.4G2] (Biotin) used in Flow cytometry (FACS). GTX01453-02

Cat. No. GTX01453-02

Reference 283

Host

 Rat

Clonality

 Monoclonal

Clone Name

 2.4G2

Isotype

 IgG2b

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($239)
See all CD16 + CD32 products
PRODUCT

Summary

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc receptor, IgG, low affinity IIb , Fc receptor, IgG, low affinity III , AI528646 , CD32 , F630109E10Rik , Fc[g]RII , FcgRII , Fcgr2 , Fcgr2a , Fcr-2 , Fcr-3 , Ly-17 , Ly-m20 , LyM-1 , Lym-1 , fcRII , CD16

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD16 + CD32 antibody [2.4G2] (Biotin) used in Flow cytometry (FACS). GTX01453-02

GTX01453-02 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01453-02 CD16 + CD32 antibody [2.4G2] (Biotin).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

REFERENCE

Clone Reference

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Patta I et al. Nucleic Acids Res 2020; 48 (11):5873-5890 Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.
Wilhelmson AS et al. Front Immunol 2020; 11:1342 Androgen Receptors in Epithelial Cells Regulate Thymopoiesis and Recent Thymic Emigrants in Male Mice.
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