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CD16 + CD32 antibody [2.4G2] (FITC)

Anti-CD16 + CD32 antibody [2.4G2] (FITC) used in Flow cytometry (FACS). GTX01453-06

Cat No. GTX01453-06

Host

Rat

Clonality

Monoclonal

Clone Name

2.4G2

Isotype

IgG2b

Application

FACS

Reactivity

Mouse
Package
100 μg ($159)
PRODUCT

Summary

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fc receptor, IgG, low affinity IIb , Fc receptor, IgG, low affinity III , AI528646 , CD32 , F630109E10Rik , Fc[g]RII , FcgRII , Fcgr2 , Fcgr2a , Fcr-2 , Fcr-3 , Ly-17 , Ly-m20 , LyM-1 , Lym-1 , fcRII , CD16

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD16 + CD32 antibody [2.4G2] (FITC) used in Flow cytometry (FACS). GTX01453-06

GTX01453-06 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01453-06 CD16 + CD32 antibody [2.4G2] (FITC).
Right panel : co-stained with CD16 + CD32 antibody [2.4G2] (FITC) and Mouse CD3e antibody (APC)
Left panel : co-stained with isotype control and Mouse CD3e antibody (APC)
antibody amount : 0.25 μg (5 μl)

REFERENCE

REVIEW

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Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 159