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CD160 antibody

Anti-CD160 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17209
Anti-CD160 antibody used in Western Blot (WB). GTX17209

Cat. No. GTX17209

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all CD160 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

K562 Cell Lysate(GTX27911)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD160 antibody was raised against a 17 amino acid peptide near the center of human CD160.The immunogen is located within amino acids 50 - 100 of CD160 .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD160 molecule , BY55 , NK1 , NK28

Background

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MH

Database

Research Area

DATA IMAGES

Anti-CD160 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17209

GTX17209 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX17209 CD160 antibody.
Working concentration : 5 μg/ml

Anti-CD160 antibody used in Western Blot (WB). GTX17209

GTX17209 WB Image

WB analysis of K562 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX17209 CD160 antibody.
Working concentration : 1 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399