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CD161 antibody [HP-3G10] (PE)

Anti-CD161 antibody [HP-3G10] (PE) used in Flow cytometry (FACS). GTX01454-08

Cat No. GTX01454-08

Host

Mouse

Clonality

Monoclonal

Clone Name

HP-3G10

Isotype

IgG1

Application

FACS

Reactivity

Human, Baboon, Chimpanzee, Rhesus Monkey
Package
100 test ($219)
PRODUCT

Summary

The HP-3G10 antibody is specific for human CD161, also known as NKR-P1A, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. CD161 exists as a homodimer which is prominently expressed on natural killer (NK) and NKT cells, where it is proposed to regulate the function of both cell types. CD161 is also found on T cell subsets, including T regulatory cells (Tregs), memory/effector CD4+ T cells, and CD8+ T cells. Th17 cells have been demonstrated to co-express CD161, as surface IL-17A+ cells are contained within the CD161+ fraction of CD4 T cells, so that CD161 (in combination with CCR6) is often used as a marker for enrichment of Th17 cells.The HP-3G10 antibody may be used for flow cytometric analysis of CD161 on NK and NKT cells, as well as on various T cell subsets. The antibody is also reported to be cross-reactive with Baboon, Chimpanzee and Rhesus CD161.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

killer cell lectin like receptor B1 , CD161 , CLEC5B , KLRB1 , NKR , NKRP1 , NKRP1A , hNKRP1A

Cellular Localization

Membrane

Background

Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD161 antibody [HP-3G10] (PE) used in Flow cytometry (FACS). GTX01454-08

GTX01454-08 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01454-08 CD161 antibody [HP-3G10] (PE).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.5 μg (5 μl)

REFERENCE

REVIEW

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Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 219