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CD162 antibody [PSGL1/1601]

Anti-CD162 antibody [PSGL1/1601] used in IHC (Paraffin sections) (IHC-P). GTX34468
Anti-CD162 antibody [PSGL1/1601] used in IHC (Paraffin sections) (IHC-P). GTX34468

Cat No. GTX34468

Host

Mouse

Clonality

Monoclonal

Clone Name

PSGL1/1601

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human full-length SELPLG (CD162) protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Selectin P Ligand , Cd162 , Cla , Psgl-1 , Psgl1 , Selplg

Background

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-CD162 antibody [PSGL1/1601] used in IHC (Paraffin sections) (IHC-P). GTX34468

GTX34468 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD162 Monoclonal Antibody (PSGL1/1601).

Anti-CD162 antibody [PSGL1/1601] used in IHC (Paraffin sections) (IHC-P). GTX34468

GTX34468 IHC-P Image

Formalin-fixed, paraffin-embedded human Spleen stained with CD162 Monoclonal Antibody (PSGL1/1601).

REFERENCE

There are currently no references for CD162 antibody [PSGL1/1601] (GTX34468). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD162 antibody [PSGL1/1601] (GTX34468). Be the first to share your experience with this product.
Package List Price ($)
$ 429