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CD163 antibody [EDHu-1]

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365
Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365
Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365
Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365
Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365
Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

Cat. No. GTX42365

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EDHu-1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, Immunoassay

Reactivity

 Human, Mouse, Sheep, Bovine, Guinea pig, Pig, Rhesus Monkey
Package
100 μg ($369)
See all CD163 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent
Immunoassay Assay dependent
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Product Note

The Binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11165403

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD163 molecule , M130 , MM130 , SCARI1

Background

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

GTX42365 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42365 CD163 antibody [EDHu-1].

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

GTX42365 IHC-Fr Image

IHC-Fr staining of a human tonsil with Mouse anti Human CD163 antibody, clone EDHu-1 (GTX42365), red in A and Mouse anti Human CD21 antibody, clone LB21 (GTX74728), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

GTX42365 IHC-Fr Image

IHC-Fr staining of a human tonsil with Mouse anti Human CD163 antibody, clone EDHu-1 (GTX42365), red in A and Mouse anti Human CD21 antibody, clone LB21 (GTX74728), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

GTX42365 IHC-Fr Image

IHC-Fr staining of a human tonsil with Mouse anti Human CD163 antibody, clone EDHu-1 (GTX42365), red in A and Mouse anti Human CD21 antibody, clone LB21 (GTX74728), green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

GTX42365 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42365 CD163 antibody [EDHu-1].

Anti-CD163 antibody [EDHu-1] used in IHC (Frozen sections) (IHC-Fr). GTX42365

GTX42365 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42365 CD163 antibody [EDHu-1].

REFERENCE

Application Reference

Ye YS et al. Neuroreport 2019; 30 (5) : 331-337 Antipruritic effects of electroacupuncture on morphine-induced pruritus model mice through the TLR2/4-MyD88-NF-κB pathway.
Application : FACS
Reactivity : Mouse
Yeh YM et al. Clin Cancer Res 2017; (Epub) The c.1085A>G genetic variant of CSF1R gene regulates tumor immunity by altering the proliferation, polarization, and function of macrophages.
Application : ICC/IF
Reactivity : Human

Clone Reference

Xu Z et al. Oncol Lett 2018; 15 (5):6519-6526 High expression of B7-H3 and CD163 in cancer tissues indicates malignant clinicopathological status and poor prognosis of patients with urothelial cell carcinoma of the bladder.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369