CD166 antibody [4H9A5]

Cat. No. GTX60798

Host	Mouse
Clonality	Monoclonal
Clone Name	4H9A5
Isotype	IgG2b
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μg ($409)

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Datasheet
Document

View product citations for antibody GTX60798 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD166 (AA: 48-216) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activated leukocyte cell adhesion molecule , CD166 , MEMD

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Database

Gene ID: 214 ALCAM
UniProt: Q13740 ALCAM

Research Area

DATA IMAGES

GTX60798 WB Image

WB analysis of NIH3T3 cell lysate using GTX60798 CD166 antibody [4H9A5].

GTX60798 ELISA Image

ELISA analysis of antigen using GTX60798 CD166 antibody [4H9A5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60798 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60798 CD166 antibody [4H9A5].

GTX60798 FACS Image

FACS analysis of Jurkat cells using GTX60798 CD166 antibody [4H9A5].
Green : CD166
Red : negative control

GTX60798 IHC-P Image

IHC-P analysis of prostate cancer tissue using GTX60798 CD166 antibody [4H9A5].

GTX60798 WB Image

WB analysis of HEK293 (1) and CD166(AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate using GTX60798 CD166 antibody [4H9A5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 409

Qty

CD166 antibody [4H9A5]

Cat. No. GTX60798

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX60798 WB Image

GTX60798 ELISA Image

GTX60798 IHC-P Image

GTX60798 FACS Image

GTX60798 IHC-P Image

GTX60798 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.