APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human CD166 (AA: 48-216) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
activated leukocyte cell adhesion molecule , CD166 , MEMD
Cellular Localization
Cell membrane,Cell projection, dendrite
Background
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
Database
Research Area
DATA IMAGES
![Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782](/templates/default/js/lightbox/images/loading.gif "Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782")
|
GTX60782 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Blue: DRAQ5 fluorescent DNA dye
|
|
|
GTX60782 IHC-P Image
IHC-P analysis of bladder cancer tissue using GTX60782 CD166 antibody [8E12C7].
|
|
|
GTX60782 ELISA Image
ELISA analysis of antigen using GTX60782 CD166 antibody [8E12C7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
|
|
|
GTX60782 IHC-P Image
IHC-P analysis of cervical cancer tissue using GTX60782 CD166 antibody [8E12C7].
|
|
|
GTX60782 FACS Image
FACS analysis of JURKAT cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Red : negative control
|
|
|
GTX60782 WB Image
WB analysis of HEK293 (1) and CD166(AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate using GTX60782 CD166 antibody [8E12C7].
|
REFERENCE
There are currently no references for CD166 antibody [8E12C7] (GTX60782). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD166 antibody [8E12C7] (GTX60782). Be the first to share your experience with this product.
![Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782](/upload/website/prouct_img/small/GTX60782/GTX60782_20170912_ICCIF_18121411_408.jpg "Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782")
![Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782](/upload/website/prouct_img/small/GTX60782/GTX60782_20170912_IHC-P_18121411_498.jpg "Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782")
![Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782](/upload/website/prouct_img/small/GTX60782/GTX60782_20170912_ELISA_18121411_439.jpg "Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782")
![Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782](/upload/website/prouct_img/small/GTX60782/GTX60782_20170912_IHC-P_1_18121411_712.jpg "Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782")
![Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782](/upload/website/prouct_img/small/GTX60782/GTX60782_20170912_FACS_18121411_133.jpg "Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782")
![Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782](/upload/website/prouct_img/small/GTX60782/GTX60782_20170912_WB_18121411_548.jpg "Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782")
![Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782](/upload/website/prouct_img/normal/GTX60782/GTX60782_20170912_ICCIF_18121411_408.jpg "Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782")
![Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782](/upload/website/prouct_img/normal/GTX60782/GTX60782_20170912_IHC-P_18121411_498.jpg "Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782")
![Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782](/upload/website/prouct_img/normal/GTX60782/GTX60782_20170912_ELISA_18121411_439.jpg "Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782")
![Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782](/upload/website/prouct_img/normal/GTX60782/GTX60782_20170912_IHC-P_1_18121411_712.jpg "Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782")
![Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782](/upload/website/prouct_img/normal/GTX60782/GTX60782_20170912_FACS_18121411_133.jpg "Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782")
![Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782](/upload/website/prouct_img/normal/GTX60782/GTX60782_20170912_WB_18121411_548.jpg "Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782")