GeneTex
United States (US)

CD166 antibody [8E12C7]

GTX60782_20170912_ICCIF_18121411_408.jpg
GTX60782_20170912_IHC-P_18121411_498.jpg
GTX60782_20170912_ELISA_18121411_439.jpg
GTX60782_20170912_IHC-P_1_18121411_712.jpg
GTX60782_20170912_FACS_18121411_133.jpg
GTX60782_20170912_WB_18121411_548.jpg

Cat No. GTX60782

Host Mouse
Clonality Monoclonal
Clone Name 8E12C7
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD166 (AA: 48-216) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MEMD Antibody , ALCAM Antibody

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX60782_20170912_ICCIF_18121411_408.jpg

GTX60782 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Blue: DRAQ5 fluorescent DNA dye

GTX60782_20170912_IHC-P_18121411_498.jpg

GTX60782 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60782 CD166 antibody [8E12C7].

GTX60782_20170912_ELISA_18121411_439.jpg

GTX60782 ELISA Image

ELISA analysis of antigen using GTX60782 CD166 antibody [8E12C7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60782_20170912_IHC-P_1_18121411_712.jpg

GTX60782 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60782 CD166 antibody [8E12C7].

GTX60782_20170912_FACS_18121411_133.jpg

GTX60782 FACS Image

FACS analysis of JURKAT cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Red : negative control

GTX60782_20170912_WB_18121411_548.jpg

GTX60782 WB Image

WB analysis of HEK293 (1) and CD166(AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate using GTX60782 CD166 antibody [8E12C7].

Package List Price ($)
$ 319