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CD166 antibody [8E12C7]

Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782
Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782
Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782
Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782
Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782
Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782

Cat No. GTX60782

Host Mouse
Clonality Monoclonal
Clone Name 8E12C7
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human
Package
100 μg ($319)
See all CD166 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD166 (AA: 48-216) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Activated Leukocyte Cell Adhesion Molecule,Cd166,Memd,Alcam

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782

GTX60782 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Blue: DRAQ5 fluorescent DNA dye
Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782

GTX60782 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60782 CD166 antibody [8E12C7].
Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782

GTX60782 ELISA Image

ELISA analysis of antigen using GTX60782 CD166 antibody [8E12C7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782

GTX60782 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60782 CD166 antibody [8E12C7].
Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782

GTX60782 FACS Image

FACS analysis of JURKAT cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Red : negative control
Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782

GTX60782 WB Image

WB analysis of HEK293 (1) and CD166(AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate using GTX60782 CD166 antibody [8E12C7].
REFERENCE
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REVIEW
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Package List Price ($)
$ 319