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CD166 antibody [8E12C7]

Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782
Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782
Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782
Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782
Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782
Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782

Cat No. GTX60782

Host

Mouse

Clonality

Monoclonal

Clone Name

8E12C7

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD166 (AA: 48-216) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

activated leukocyte cell adhesion molecule , CD166 , MEMD

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD166 antibody [8E12C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60782

GTX60782 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Blue: DRAQ5 fluorescent DNA dye

Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782

GTX60782 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60782 CD166 antibody [8E12C7].

Anti-CD166 antibody [8E12C7] used in ELISA (ELISA). GTX60782

GTX60782 ELISA Image

ELISA analysis of antigen using GTX60782 CD166 antibody [8E12C7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-CD166 antibody [8E12C7] used in IHC (Paraffin sections) (IHC-P). GTX60782

GTX60782 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60782 CD166 antibody [8E12C7].

Anti-CD166 antibody [8E12C7] used in Flow cytometry (FACS). GTX60782

GTX60782 FACS Image

FACS analysis of JURKAT cells using GTX60782 CD166 antibody [8E12C7].
Green : CD166
Red : negative control

Anti-CD166 antibody [8E12C7] used in Western Blot (WB). GTX60782

GTX60782 WB Image

WB analysis of HEK293 (1) and CD166(AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate using GTX60782 CD166 antibody [8E12C7].

REFERENCE

There are currently no references for CD166 antibody [8E12C7] (GTX60782). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD166 antibody [8E12C7] (GTX60782). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379