APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| 4μg/ml antibody / 2x10⁵ K562 cells |
| Assay dependent |
| Assay dependent |
|
Note :
WB
A non-reduced sample treatment and 5 % SDS-Page was used. The mature protein has a band size of 100 kDa and the soluble form 30 kDa.
IHC-P
Tissue sections were pretreated by heating in a rice steamer in citrate buffer for 10 minutes for antigen retrieval. Tissue sections were blocked with normal horse serum.
IHC-Fr
Tissue sections were fixed in acetone
IP
Radioactive labelled KG1a cells were lysed and precipitated with 1 μg of antibody coupled to Protein G Sepharose 4 Fast Flow beads.
Neutralizing/Inhibition
Antibody AZN-L50 functions as an blocking antibody for homotypic ALCAM-ALCAM interaction.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody stains the extracellular domain of ALCAM. The monoclonal antibody AZN-L50 completely blocks homophilic ALCAM-ALCAM interaction.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
CD166 expressing K562 cells
Purification
Purified IgG2a
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ALCAM,CD166,MEMD,activated leukocyte cell adhesion molecule
Cellular Localization
Cell membrane,Cell projection,Axon,Dendrite,Secreted
Background
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
Database
Research Area
REFERENCE
There are currently no references for CD166 antibody [AZN-L50] (GTX54415). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD166 antibody [AZN-L50] (GTX54415). Be the first to share your experience with this product.
