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CD166 antibody, N-term

Anti-CD166 antibody, N-term used in Western Blot (WB). GTX81961
Anti-CD166 antibody, N-term used in Flow cytometry (FACS). GTX81961
Anti-CD166 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81961

Cat. No. GTX81961

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all CD166 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human CD166.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activated leukocyte cell adhesion molecule , CD166 , MEMD

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD166 antibody, N-term used in Western Blot (WB). GTX81961

GTX81961 WB Image

WB analysis of NCI-H460 cell lysate (35ug/lane) using GTX81961 CD166 antibody, N-term.

Anti-CD166 antibody, N-term used in Flow cytometry (FACS). GTX81961

GTX81961 FACS Image

FACS analysis of NCI-H292 cells using GTX81961 CD166 antibody, N-term.
Top histogram : negative control
Bottom histogram : NCI-H292 cells

Anti-CD166 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81961

GTX81961 IHC-P Image

IHC-P analysis of human brain tissue using GTX81961 CD166 antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399