CD168 / RHAMM antibody [N1N2], N-term

Name: CD168 / RHAMM antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX121502
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Western Blot (WB). GTX121502

Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX121502

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunohistochemistry (IHC). GTX121502

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121502

Cat. No. GTX121502

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all CD168 / RHAMM products

View product citations for antibody GTX121502 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IP	1:100-1:500
IHC	Assay dependent

Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Human ESC

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CD168 / RHAMM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hyaluronan mediated motility receptor , CD168 , IHABP , RHAMM

Cellular Localization

Cell surface , Cytoplasm

Background

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

Gene ID: 3161 HMMR
UniProt: O75330 HMMR

Research Area

DATA IMAGES

GTX121502 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX121502 diluted at 1:1000

GTX121502 IHC-P Image

RHAMM antibody [N1N2], N-term detects RHAMM protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RHAMM antibody [N1N2], N-term (GTX121502) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121502 IP Image

Immunoprecipitation of RHAMM protein from 293T whole cell extracts using 5 μg of RHAMM antibody [N1N2], N-term (GTX121502).
Western blot analysis was performed using RHAMM antibody [N1N2], N-term (GTX121502).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX121502 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RHAMM(GTX121502) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX121502 IHC Image

The data was published in the journal Oncotarget in 2017. PMID: 29152086

GTX121502 WB Image

The data was published in the journal Oncotarget in 2017. PMID: 29152086

GTX121502 ICC/IF Image

The data was published in the journal Oncotarget in 2017. PMID: 29152086

REFERENCE

Application Reference

Li J et al. Neoplasia 2018; 20 (5) : 456-466 Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma.

Application : WB

Reactivity : Human

Li J et al. Oncotarget 2017; 8 (50) : 87353-87363 COX-2/sEH dual inhibitor PTUPB suppresses glioblastoma growth by targeting epidermal growth factor receptor and hyaluronan mediated motility receptor.

Application : WB

Reactivity : Human

Huang M et al. Oncotarget 2017; 8 (43) : 74479-74493 FAM83D, a microtubule-associated protein, promotes tumor growth and progression of human gastric cancer.

Application : WB

Reactivity : Human

Li J et al. Oncotarget 2017; 8 : 87353-87363 COX-2/sEH dual inhibitor PTUPB suppresses glioblastoma growth by targeting epidermal growth factor receptor and hyaluronan mediated motility receptor.

Application : WB, IHC-P

Reactivity : Human

Lee YL et al. Am J Pathol 2017; 187 (9) : 1945-1959 elucidating mechanisms of bladder repair after hyaluronan instillation in *-induced ulcerative cystitis in animal model.

Application : WB, IHC

Reactivity : Rat

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