GeneTex

  • Country / Location Selection

United States (US)

CD168 / RHAMM antibody [N1N2], N-term

Orthogonal
Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX121502
Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502
Anti-CD168 / RHAMM antibody [N1N2], N-term used in Western Blot (WB). GTX121502
Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

Cat. No. GTX121502

Reference 5
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD168 / RHAMM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Human ESC

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CD168 / RHAMM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163915

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hyaluronan mediated motility receptor , CD168 , IHABP , RHAMM

Cellular Localization

Cell surface , Cytoplasm

Background

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX121502

GTX121502 IP Image

Immunoprecipitation of RHAMM protein from 293T whole cell extracts using 5 μg of RHAMM antibody [N1N2], N-term (GTX121502).
Western blot analysis was performed using RHAMM antibody [N1N2], N-term (GTX121502).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

GTX121502 IHC-P Image

RHAMM antibody [N1N2], N-term detects RHAMM protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RHAMM antibody [N1N2], N-term (GTX121502) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Western Blot (WB). GTX121502

GTX121502 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX121502 diluted at 1:1000

Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

GTX121502 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RHAMM(GTX121502) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Li J et al. Neoplasia 2018; 20 (5) : 456-466 Targeting Long Noncoding RNA HMMR-AS1 Suppresses and Radiosensitizes Glioblastoma.
Application : WB
Reactivity : Human
Li J et al. Oncotarget 2017; 8 (50) : 87353-87363 COX-2/sEH dual inhibitor PTUPB suppresses glioblastoma growth by targeting epidermal growth factor receptor and hyaluronan mediated motility receptor.
Application : WB
Reactivity : Human
Huang M et al. Oncotarget 2017; 8 (43) : 74479-74493 FAM83D, a microtubule-associated protein, promotes tumor growth and progression of human gastric cancer.
Application : WB
Reactivity : Human
Li J et al. Oncotarget 2017; 8 : 87353-87363 COX-2/sEH dual inhibitor PTUPB suppresses glioblastoma growth by targeting epidermal growth factor receptor and hyaluronan mediated motility receptor.
Application : WB, IHC-P
Reactivity : Human
Lee YL et al. Am J Pathol 2017; 187 (9) : 1945-1959 elucidating mechanisms of bladder repair after hyaluronan instillation in *-induced ulcerative cystitis in animal model.
Application : WB, IHC
Reactivity : Rat
Submit a Reference

REVIEW

CD168 / RHAMM antibody [N1N2], N-term Cat. No. GTX121502

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunohistochemistry (IHC). GTX121502
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29152086
Anti-CD168 / RHAMM antibody [N1N2], N-term used in Western Blot (WB). GTX121502
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29152086
Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121502
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29152086
Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502
Date : Anonymous submitted on 02-Dec-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast carcinoma tissue
Fixation : 10% formalin
Permeabilization /Antigen retrieval : CC1
Primary Antibody : 1:250, 37°C, 1Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169