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CD168 / RHAMM antibody [N1N2], N-term

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX121502
Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502
Anti-CD168 / RHAMM antibody [N1N2], N-term used in Western Blot (WB). GTX121502
Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

Cat No. GTX121502

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

human ESC

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CD168 / RHAMM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163915

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hyaluronan mediated motility receptor , CD168 , IHABP , RHAMM

Cellular Localization

Cell surface , Cytoplasm

Background

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX121502

GTX121502 IP Image

Immunoprecipitation of RHAMM protein from 293T whole cell extracts using 5 μg of RHAMM antibody [N1N2], N-term (GTX121502).
Western blot analysis was performed using RHAMM antibody [N1N2], N-term (GTX121502).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

GTX121502 IHC-P Image

RHAMM antibody [N1N2], N-term detects RHAMM protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RHAMM antibody [N1N2], N-term (GTX121502) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD168 / RHAMM antibody [N1N2], N-term used in Western Blot (WB). GTX121502

GTX121502 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX121502 diluted at 1:1000

Anti-CD168 / RHAMM antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121502

GTX121502 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RHAMM(GTX121502) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

CD168 / RHAMM antibody [N1N2], N-term Cat No. GTX121502

Rating ( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29152086
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29152086
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29152086
Date : Anonymous submitted on 02-Dec-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast carcinoma tissue
Fixation : 10% formalin
Permeabilization /Antigen retrieval : CC1
Primary Antibody : 1:250, 37°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169