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CD169 antibody

Anti-CD169 antibody used in Western Blot (WB). GTX131703
Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703
Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703

Cat No. GTX131703

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CD169. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sialic acid binding Ig like lectin 1,CD169,SIGLEC-1,SN

Background

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-CD169 antibody used in Western Blot (WB). GTX131703

GTX131703 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD169 antibody (GTX131703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703

GTX131703 IHC-P Image

CD169 antibody detects CD169 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
CD169 stained by CD169 antibody (GTX131703) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703

GTX131703 IHC-P Image

CD169 antibody detects CD169 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
CD169 stained by CD169 antibody (GTX131703) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169