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CD169 antibody

Anti-CD169 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131703
Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703
Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703

Cat. No. GTX131703

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD169 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD169. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886515

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sialic acid binding Ig like lectin 1 , CD169 , SIGLEC-1 , SN

Background

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD169 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131703

GTX131703 ICC/IF Image

CD169 antibody detects CD169 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD169 stained by CD169 antibody (GTX131703) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703

GTX131703 IHC-P Image

CD169 antibody detects CD169 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
CD169 stained by CD169 antibody (GTX131703) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD169 antibody used in IHC (Paraffin sections) (IHC-P). GTX131703

GTX131703 IHC-P Image

CD169 antibody detects CD169 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
CD169 stained by CD169 antibody (GTX131703) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jia Y et al. Adv Sci (Weinh) 2023; 10 (7) : e2204565 Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169