APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄
Preservative
0.05mg Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E. coli-derived human CD16 recombinant protein (Position: Q101-D166).
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fc fragment of IgG receptor IIIa , CD16 , CD16A , FCG3 , FCGR3 , FCGRIII , FCR-10 , FCRIII , FCRIIIA , IGFR3 , IMD20
Cellular Localization
Cell membrane , Secreted
Background
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX03379 IHC-P Image
IHC-P analysis of human rectal cancer tissue using GTX03379 CD16a antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Dilution : 2 μg/ml
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GTX03379 ICC/IF Image
ICC/IF analysis of K562 cells using GTX03379 CD16a antibody.
Antigen retireval : IHC enzyme antigen retrieval reagent for 15min.
Dilution : 5 μg/mL
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GTX03379 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX03379 CD16a antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Dilution : 2 μg/ml
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GTX03379 FACS Image
FACS analysis of human PBMC cells using GTX03379 CD16a antibody.
Blue : Primary antibody
Green : Isotype control>
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells
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GTX03379 IHC-P Image
IHC-P analysis of human rectal cancer tissue using GTX03379 CD16a antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Dilution : 2 μg/ml
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REFERENCE
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REVIEW
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