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CD177 antibody [MEM-166]

Cat. No. GTX75711

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-166

Isotype

 IgG1

Application

 WB, FACS, IP

Reactivity

 Human, Rhesus Monkey
Package
100 μg ($399)
See all CD177 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS 1/50-1/100
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Product Note

This antibody recognizes human CD177 (neutrophil glycoprotein NB1).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human granulocytes.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_376981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD177 molecule , HNA-2a , HNA2A , NB1 , NB1 GP , PRV-1 , PRV1

Cellular Localization

Cell membrane , Secreted

Background

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]

Database

Research Area

REFERENCE

Application Reference

Jankowska AM et al. Haematologica 2011; 96 (7) : 954-62 Loss of expression of neutrophil proteinase-3: a factor contributing to thrombotic risk in paroxysmal nocturnal hemoglobinuria.
Application : FACS
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399