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CD18 antibody [MEM-148] (Azide free)

Anti-CD18 antibody [MEM-148] (Azide free) used in Flow cytometry (FACS). GTX75792
Anti-CD18 antibody [MEM-148] (Azide free) used in Flow cytometry (FACS). GTX75792

Cat. No. GTX75792

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-148

Isotype

 IgG1

Application

 WB, FACS, IP, Functional Assay

Reactivity

 Human
Package
500 μg ($469)
See all CD18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS 1/20-1/200
IP Assay dependent
Functional Assay Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Product Note

This antibody recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peripheral blood mononuclear cells.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_377036

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 2 , CD18 , LAD , LCAMB , LFA-1 , MAC-1 , MF17 , MFI7

Cellular Localization

Cell membrane

Background

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-CD18 antibody [MEM-148] (Azide free) used in Flow cytometry (FACS). GTX75792

GTX75792 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX75792 CD18 antibody [MEM-148] (Azide free).

Anti-CD18 antibody [MEM-148] (Azide free) used in Flow cytometry (FACS). GTX75792

GTX75792 FACS Image

FACS analysis of human peripheral blood monocytes using GTX75792 CD18 antibody [MEM-148] (Azide free).

REFERENCE

Application Reference

Li N et al. J Immunol 2013; 190 (8) : 4371-81 Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
Application : Functional Assay
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 469
Custom Conjugations