GeneTex
United States (US)

CD18 antibody [MEM-148]

CD18-antibody-MEM-148__GTX28220-1_18121409_285.jpg
CD18-antibody-MEM-148__GTX28220-2_18121409_550.jpg

Cat No. GTX28220

Host Mouse
Clonality Monoclonal
Clone Name MEM-148
Isotype IgG1
Application WB, FACS, IP, Functional Assay
Reactivity Human
APPLICATION

Application Note

FACS: Use at an assay dependant concentration. The antibody is excellent marker of activated myeloid cells. FuncS: Use at an assay dependant dilution. The antibody induces homotypic cell aggregation and high-affinity LFA-1 conformation inresting leukocytes. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

85 kDa. ( Note )

Specificity/Sensitivity

Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tissue / cell preparation (Human). (Peripheral blood mononuclear cells).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
CD18-antibody-MEM-148__GTX28220-1_18121409_285.jpg

GTX28220 WB Image

Western Blot analysis of PMA-activated monocytes (Fig. 1B), using anti-human CD18 (MEM-148) (GTX28220). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

CD18-antibody-MEM-148__GTX28220-2_18121409_550.jpg

GTX28220 WB Image

Western Blot analysis of PMA-activated neutrophils (Fig. 1A), using anti-human CD18 (MEM-148) (GTX28220). The antibody MEM-148 recognizes the lower 65 to 70 kDa zone (activation marker) as well as the upper 78 to 96 kDa zone (CD18).

REFERENCE
Package List Price ($)
$ 319