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CD18 antibody [MEM-48] (PE)

Anti-CD18 antibody [MEM-48] (PE) used in IHC (Paraffin sections) (IHC-P). GTX79945
Anti-CD18 antibody [MEM-48] (PE) used in Flow cytometry (FACS). GTX79945
Anti-CD18 antibody [MEM-48] (PE) used in Western Blot (WB). GTX79945

Cat. No. GTX79945

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-48

Isotype

 IgG1

Application

 WB, IHC-P, FACS, Blocking

Reactivity

 Human, Mouse
Package
100 test ($399)
See all CD18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
FACS 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Blocking Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Product Note

This antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin).
We do not recommend use of this product for Dog samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Leukocytes of a patient suffering from a LGL-type leukemia.

Purification

Purified by size-exclusion chromatography

Conjugation

Phycoerythrin (PE)

RRID

AB_11163653

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD18 , ITGB2 , LAD , LCAMB , LFA1 , MAC1 , MF17 , MFI7 , integrin subunit beta 2 , Integrin beta2

Cellular Localization

Membrane

Background

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-CD18 antibody [MEM-48] (PE) used in IHC (Paraffin sections) (IHC-P). GTX79945

GTX79945 IHC-P Image

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Anti-CD18 antibody [MEM-48] (PE) used in Flow cytometry (FACS). GTX79945

GTX79945 FACS Image

Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.

Anti-CD18 antibody [MEM-48] (PE) used in Western Blot (WB). GTX79945

GTX79945 WB Image

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line. Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

REFERENCE

Clone Reference

Tran DQ et al. J Immunol 2009; 182 (5):2929-38 Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
Application : Blocking
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399