APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:10-1:100 |
| 0.5 μg/test |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human Beta 2 Integrin
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
integrin subunit beta 2 , CD18 , LAD , LCAMB , LFA-1 , MAC-1 , MF17 , MFI7
Cellular Localization
Cell membrane
Background
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
![Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770](/templates/default/js/lightbox/images/loading.gif "Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770")
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GTX15770 FACS Image
FACS analysis of PBMC cells using GTX15770 CD18 antibody [TS1/18] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4ºC
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GTX15770 FACS Image
FACS analysis of Jurkat cells using GTX15770 CD18 antibody [TS1/18] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX15770 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX15770 CD18 antibody [TS1/18].
Right : Primary antibody
Left : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
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GTX15770 ICC/IF Image
ICC/IF analysis of THP-1 cells using GTX15770 CD18 antibody [TS1/18].
Right : Primary antibody
Left : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
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GTX15770 ICC/IF Image
ICC/IF analysis of Hela cells using GTX15770 CD18 antibody [TS1/18]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
|
REFERENCE
There are currently no references for CD18 antibody [TS1/18] (GTX15770). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD18 antibody [TS1/18] (GTX15770). Be the first to share your experience with this product.
![Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770](/upload/website/prouct_img/small/GTX15770/GTX15770_114_FACS_w_23060620_288.webp "Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770](/upload/website/prouct_img/small/GTX15770/GTX15770_113_FACS_w_23060620_708.webp "Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770](/upload/website/prouct_img/small/GTX15770/GTX15770_300_ICC-IF_w_23060620_354.webp "Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770](/upload/website/prouct_img/small/GTX15770/GTX15770_301_ICC-IF_w_23060620_748.webp "Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770](/upload/website/prouct_img/small/GTX15770/GTX15770_299_ICC-IF_w_23060620_812.webp "Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770](/upload/website/prouct_img/normal/GTX15770/GTX15770_114_FACS_w_23060620_288.webp "Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770](/upload/website/prouct_img/normal/GTX15770/GTX15770_113_FACS_w_23060620_708.webp "Anti-CD18 antibody [TS1/18] used in Flow cytometry (FACS). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770](/upload/website/prouct_img/normal/GTX15770/GTX15770_300_ICC-IF_w_23060620_354.webp "Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770](/upload/website/prouct_img/normal/GTX15770/GTX15770_301_ICC-IF_w_23060620_748.webp "Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770")
![Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770](/upload/website/prouct_img/normal/GTX15770/GTX15770_299_ICC-IF_w_23060620_812.webp "Anti-CD18 antibody [TS1/18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15770")