GeneTex
United States (US)

CD18 antibody [TS1/18]

GTX15770_113_FACS_19011519_294.jpg
GTX15770_299_ICC-IF_19011519_752.jpg
GTX15770_301_ICC-IF_19011519_869.jpg
GTX15770_300_ICC-IF_19011519_905.jpg
GTX15770_114_FACS_19011519_574.jpg

Cat No. GTX15770

Host Mouse
Clonality Monoclonal
Clone Name TS1/18
Isotype IgG1
Application ICC/IF, FACS, IHC, Inhibition assay, Neut
Reactivity Human, Mouse, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:10-1:100
FACS 0.5 μg/test
IHC Assay dependent
Inhibition assay Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Beta 2 Integrin

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Beta 2,Cd18,Lad,Lcamb,Lfa-1,Mac-1,Mf17,Mfi7,Itgb2

Cellular Localization

Cell membrane,Membrane raft

Background

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES
GTX15770_113_FACS_19011519_294.jpg

GTX15770 FACS Image

FACS analysis of Jurkat cells using GTX15770 CD18 antibody [TS1/18] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX15770_299_ICC-IF_19011519_752.jpg

GTX15770 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15770 CD18 antibody [TS1/18]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

GTX15770_301_ICC-IF_19011519_869.jpg

GTX15770 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX15770 CD18 antibody [TS1/18].
Right : Primary antibody
Left : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

GTX15770_300_ICC-IF_19011519_905.jpg

GTX15770 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX15770 CD18 antibody [TS1/18].
Right : Primary antibody
Left : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

GTX15770_114_FACS_19011519_574.jpg

GTX15770 FACS Image

FACS analysis of PBMC cells using GTX15770 CD18 antibody [TS1/18] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4°C

SDS
PBS.pdf
Package List Price ($)
$ 319