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CD18 antibody [YFC118.3]

Anti-CD18 antibody [YFC118.3] used in Flow cytometry (FACS). GTX76238

Cat No. GTX76238

Host

Rat

Clonality

Monoclonal

Clone Name

YFC118.3

Isotype

IgG2b

Application

IHC-Fr, FACS, IP

Reactivity

Human, Dog, Guinea pig
Package
100 μg ($299)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-Fr 1/1,000-1/5,000
FACS 1/50-1/100
IP Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Specificity/Sensitivity

Clone YFC118.3 is a strong reactivity with Leucocytes (platelets negative).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human neutrophils.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Integrin Subunit Beta 2 , Cd18 , Lad , Lcamb , Lfa-1 , Mac-1 , Mf17 , Mfi7 , Itgb2

Background

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-CD18 antibody [YFC118.3] used in Flow cytometry (FACS). GTX76238

GTX76238 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX76238 CD18 antibody [YFC118.3].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 299