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CD18 antibody

Anti-CD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX54372
Anti-CD18 antibody used in Western Blot (WB). GTX54372
Anti-CD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54372
Anti-CD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX54372
Anti-CD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX54372

Cat. No. GTX54372

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human ITGB2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 2 , CD18 , LAD , LCAMB , LFA-1 , MAC-1 , MF17 , MFI7

Cellular Localization

Cell membrane

Background

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-CD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX54372

GTX54372 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX54372 CD18 antibody.
Dilution : 1:100

Anti-CD18 antibody used in Western Blot (WB). GTX54372

GTX54372 WB Image

WB analysis of various sample lysates using GTX54372 CD18 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54372

GTX54372 ICC/IF Image

ICC/IF analysis of Raw264.7 cells using GTX54372 CD18 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX54372

GTX54372 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX54372 CD18 antibody.
Dilution : 1:100

Anti-CD18 antibody used in IHC (Paraffin sections) (IHC-P). GTX54372

GTX54372 IHC-P Image

IHC-P analysis of rat lung tissue using GTX54372 CD18 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Chen SH et al. Front Neurosci 2023; 17 : 1172740 Adipose-derived stem cells modulate neuroinflammation and improve functional recovery in chronic constriction injury of the rat sciatic nerve.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399