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CD19 antibody [1D3] (PE)

Anti-CD19 antibody [1D3] (PE) used in Flow cytometry (FACS). GTX00554-08

Cat. No. GTX00554-08

Host

 Rat

Clonality

 Monoclonal

Clone Name

 1D3

Isotype

 IgG3a

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($339)
See all CD19 products
PRODUCT

Summary

The 1D3 antibody reacts with mouse CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 and CD81, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells. The 1D3 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse CD19-transfected cell line

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD19 antigen , AW495831

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [1D3] (PE) used in Flow cytometry (FACS). GTX00554-08

GTX00554-08 FACS Image

FACS analysis of C57Bl/6 splenocytes using GTX00554-08 CD19 antibody [1D3] (PE).
Solid line : Primary antibody
Dashed line : PE rat IgG2a isotype control
Antibody amount : 0.125 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339