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CD19 antibody [C1C3]

Anti-CD19 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101512
Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512
Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512
Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512
Anti-CD19 antibody [C1C3] used in Flow cytometry (FACS). GTX101512
Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

Cat. No. GTX101512

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse leukocyte

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731496

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD19 molecule , B4 , CVID3

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101512

GTX101512 ICC/IF Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512

GTX101512 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512

GTX101512 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000.

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

GTX101512 IHC-P Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD19 antibody [C1C3] used in Flow cytometry (FACS). GTX101512

GTX101512 FACS Image

CD19 antibody [C1C3] (GTX101512) detects CD19 protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD19 antibody [C1C3] (GTX101512) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

GTX101512 IHC-P Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yasuda T et al. Sci Rep 2021; 11 (1) : 5524 Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells.
Application : WB
Reactivity : Mouse
Kurimoto C et al. Cancer Sci 2020; (Epub) Predictive and sensitive biomarkers for thyroid dysfunctions during treatment with immune-checkpoint inhibitors.
Application : IHC-P
Reactivity : Mouse
Bottero V et al. J Virol 2013; 87 (21) : 11806-20 Kaposi's sarcoma-associated herpesvirus-positive primary effusion lymphoma tumor formation in NOD/SCID mice is inhibited by neomycin and neamine blocking angiogenin's nuclear translocation.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169