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CD19 antibody [C1C3]

Anti-CD19 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101512
Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512
Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512
Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512
Anti-CD19 antibody [C1C3] used in Flow cytometry (FACS). GTX101512
Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

Cat. No. GTX101512

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse leukocyte

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human CD19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731496

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD19 molecule , B4 , CVID3

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101512

GTX101512 ICC/IF Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512

GTX101512 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512

GTX101512 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000.

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

GTX101512 IHC-P Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD19 antibody [C1C3] used in Flow cytometry (FACS). GTX101512

GTX101512 FACS Image

CD19 antibody [C1C3] (GTX101512) detects CD19 protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD19 antibody [C1C3] (GTX101512) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

GTX101512 IHC-P Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yasuda T et al. Sci Rep 2021; 11 (1) : 5524 Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells.
Application : WB
Reactivity : Mouse
Kurimoto C et al. Cancer Sci 2020; (Epub) Predictive and sensitive biomarkers for thyroid dysfunctions during treatment with immune-checkpoint inhibitors.
Application : IHC-P
Reactivity : Mouse
Bottero V et al. J Virol 2013; 87 (21) : 11806-20 Kaposi's sarcoma-associated herpesvirus-positive primary effusion lymphoma tumor formation in NOD/SCID mice is inhibited by neomycin and neamine blocking angiogenin's nuclear translocation.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169