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CD19 antibody [C1C3]

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512
Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512
Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512
Anti-CD19 antibody [C1C3] used in Flow cytometry (FACS). GTX101512
Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512
Anti-CD19 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101512

Cat No. GTX101512

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Raji , mouse leukocyte

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731496

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD19 molecule , B4 , CVID3

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

GTX101512 IHC-P Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512

GTX101512 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD19 antibody [C1C3] used in Western Blot (WB). GTX101512

GTX101512 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000.

Anti-CD19 antibody [C1C3] used in Flow cytometry (FACS). GTX101512

GTX101512 FACS Image

CD19 antibody [C1C3] (GTX101512) detects CD19 protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4ºC for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD19 antibody [C1C3] (GTX101512) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-CD19 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101512

GTX101512 IHC-P Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD19 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101512

GTX101512 ICC/IF Image

CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169