GeneTex
  • Country / Location Selection

United States (US)

CD19 antibody [HIB19] (PerCP-Cy5.5)

Anti-CD19 antibody [HIB19] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01455-11

Cat No. GTX01455-11

Host

Mouse

Clonality

Monoclonal

Clone Name

HIB19

Isotype

IgG1

Application

FACS

Reactivity

Human
Package
100 test ($229)
PRODUCT

Summary

The HIB19 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21, CD81 and CD225, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The HIB19 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.025 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD19 molecule , B4 , CD19 , CVID3

Cellular Localization

Membrane

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [HIB19] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01455-11

GTX01455-11 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01455-11 CD19 antibody [HIB19] (PerCP-Cy5.5).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.125 μg (5 μl)

REFERENCE

REVIEW

There are currently no reviews for CD19 antibody [HIB19] (PerCP-Cy5.5) (GTX01455-11). Be the first to share your experience with this product.
Clone Reference