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CD19 antibody [LE-CD19]

Anti-CD19 antibody [LE-CD19] used in IHC (Paraffin sections) (IHC-P). GTX42324
Anti-CD19 antibody [LE-CD19] used in Western Blot (WB). GTX42324

Cat. No. GTX42324

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LE-CD19

Isotype

 IgG1

Application

 WB, IHC-P, FACS, IP, ELISA

Reactivity

 Human
Package
20 μg ($169)
See all CD19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/100-1/200
FACS 1/100-1/200
IP Assay dependent
ELISA Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD19 peptide CGPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD19 molecule , B4 , CVID3

Cellular Localization

Cell membrane

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [LE-CD19] used in IHC (Paraffin sections) (IHC-P). GTX42324

GTX42324 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX42324 CD19 antibody [LE-CD19].

Anti-CD19 antibody [LE-CD19] used in Western Blot (WB). GTX42324

GTX42324 WB Image

WB analysis of Raji cell lysate using GTX42324 CD19 antibody [LE-CD19].

REFERENCE

Clone Reference

Richmond BW et al. Mucosal Immunol 2020; (Epub) Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169