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CD19 antibody [LE-CD19]

Anti-CD19 antibody [LE-CD19] used in IHC (Paraffin sections) (IHC-P). GTX42325
Anti-CD19 antibody [LE-CD19] used in Western Blot (WB). GTX42325

Cat No. GTX42325

Host

Mouse

Clonality

Monoclonal

Clone Name

LE-CD19

Isotype

IgG1

Application

WB, IHC-P, FACS, IP, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1/100-1/200
FACS 1/100-1/200
IP Assay dependent
ELISA Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD19 peptide CGPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD19 molecule , B4 , CVID3

Cellular Localization

Cell membrane

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD19 antibody [LE-CD19] used in IHC (Paraffin sections) (IHC-P). GTX42325

GTX42325 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX42325 CD19 antibody [LE-CD19].

Anti-CD19 antibody [LE-CD19] used in Western Blot (WB). GTX42325

GTX42325 WB Image

WB analysis of Raji cell lysate using GTX42325 CD19 antibody [LE-CD19].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf