PRODUCT
Summary
This antibody was validated on 76 different Normal Tissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6.0, the immunostaining is markedly reduced.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Skin: A strong CD1a staining should be seen in Langerhans cells.
Negative Control
Skin: CD1a staining must be absent in squamous epithelial cells and sebaceous glands cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human CD1a protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD1a molecule , CD1 , FCB6 , HTA1 , R4 , T6
Cellular Localization
Cell membrane , Endosome membrane
Background
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
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GTX04407 IHC-P Image
IHC-P analysis of human thymic cortex tissue section using GTX04407 CD1a antibody [MSVA-001M] HistoMAX. A strong CD1a immunostaining is seen in 80 of small lymphocytes in the thymic cortex.
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GTX04407 IHC-P Image
IHC-P analysis of human skin squamous epithelium tissue section using GTX04407 CD1a antibody [MSVA-001M] HistoMAX. Strong CD1 immunostaining of Langerhans cells in squamous epithelium of the skin.
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GTX04407 IHC-P Image
IHC-P analysis of human appendix tissue section using GTX04407 CD1a antibody [MSVA-001M] HistoMAX. Lymphatic and epithelial cells of the appendix completely lack CD1a immunostaining.
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REFERENCE
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REVIEW
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