GeneTex
United States (US)

CD2 antibody [B336 (HuLy-m1)]

Cat No. GTX28670

Host Mouse
Clonality Monoclonal
Clone Name B336 (HuLy-m1)
Isotype IgG2b
Application IHC-Fr, FACS
Reactivity Human
APPLICATION

Application Note

For flow cytometry use at 1-5 mg/106 cells. For immunohistochemistry on frozen tissue sections use 10-20 μg/mL. The optimal conditionsshould be determined by the individual lab.

Calculated MW

39 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with the majority of T-cells in peripheral lymphoid tissue, cortical thymocytes, and most T-cell neoplasms This antibody reacts with the E-rosette receptor on T-cells. This receptor has a molecular weight of approximately 50 kD and is present on the surface of the majority of circulating T-cells.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 1%BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.4mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human thymocytes (3 pooled donors).

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM]

Database

Research Area

Package List Price ($)
$ 319