For flow cytometry use at 1-5 mg/106 cells. For immunohistochemistry on frozen tissue sections use 10-20 μg/mL. The optimal conditionsshould be determined by the individual lab.
This antibody reacts with the majority of T-cells in peripheral lymphoid tissue, cortical thymocytes, and most T-cell neoplasms This antibody reacts with the E-rosette receptor on T-cells. This receptor has a molecular weight of approximately 50 kD and is present on the surface of the majority of circulating T-cells.
Phosphate-buffered saline, pH 7.2, containing 1%BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.4mg/ml(Please refer to the vial label for the specific concentration.)
Human thymocytes (3 pooled donors).
Protein A affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM]