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CD20 antibody [2H7] (FITC)

Anti-CD20 antibody [2H7] (FITC) used in Flow cytometry (FACS). GTX75268

Cat. No. GTX75268

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H7

Isotype

 IgG2b

Application

 FACS

Reactivity

 Human, Rhesus Monkey
Package
100 μg ($399)
See all CD20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat-1/10

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

The epitope recognized by clone 2H7 has been mapped to the following sequence found in the large extracellular loop of human CD20: YNCEPANPSEKNSPST. Furthermore it appears that Mouse anti Human CD20 antibody, clone 2H7 only recognizes human CD20 in its native oligomeric form (Polyak et al. 2002).

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_376688

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7

Cellular Localization

Cell membrane

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD20 antibody [2H7] (FITC) used in Flow cytometry (FACS). GTX75268

GTX75268 FACS Image

Figure A. RPE conjugated mouse anti human CD19 (GTX75601) and FITC conjugated Mouse IgG2b isotype control (GTX76400). Figure B. RPE conjugated mouse anti human CD19 (GTX75601) and FITC conjugated mouse anti human CD20 (GTX75268). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes

REFERENCE

Application Reference

Kotani N et al. J Biol Chem 2012; 287 (44) : 37109-18 Fibroblast growth factor receptor 3 (FGFR3) associated with the CD20 antigen regulates the rituximab-induced proliferation inhibition in B-cell lymphoma cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399