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CD20 antibody [2H7] (PE)

Anti-CD20 antibody [2H7] (PE) used in Flow cytometry (FACS). GTX00466-08

Cat. No. GTX00466-08

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H7

Isotype

 IgG2b

Application

 FACS

Reactivity

 Human, Baboon, Chimpanzee, Cynomolgus monkey, Pig-tailed macaque, Rhesus Monkey, Squirrel monkey, Capuchin monkey
Package
100 test ($359)
See all CD20 products
PRODUCT

Summary

The 2H7 antibody reacts with human CD20, expressed by pre-B cells, resting and activated B cells, follicular dendritic cells and a small subset of T cells, but not on plasma cells. CD20 is a 33-37 kDa transmembrane protein which is differentially phosphorylated in resting and active B cells. The CD20 molecule plays a role in regulating B-cell activation.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.06 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.012 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human tonsillar B cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7

Cellular Localization

Cell membrane

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD20 antibody [2H7] (PE) used in Flow cytometry (FACS). GTX00466-08

GTX00466-08 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX00466-08 CD20 antibody [2H7] (PE).
Solid line : Primary antibody
Dashed line : PE mouse IgG2b isotype control
Antibody amount : 0.06 μg

REFERENCE

Clone Reference

Kotani N et al. J Biol Chem 2012; 287 (44):37109-18 Fibroblast growth factor receptor 3 (FGFR3) associated with the CD20 antigen regulates the rituximab-induced proliferation inhibition in B-cell lymphoma cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 359