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CD20 antibody [C2C3], C-term

Anti-CD20 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209
Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

Cat. No. GTX100209

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all CD20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

K562 , THP-1

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080937

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7

Cellular Localization

Membrane

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD20 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100209

GTX100209 IHC-P Image

CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD20 stained by CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD20 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100209

GTX100209 ICC/IF Image

CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD20 protein stained by CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

GTX100209 WB Image

Sample (30 ug of whole cell lysate)
A: K562
B: THP-1
12% SDS PAGE
GTX100209 diluted at 1:10000

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

GTX100209 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted withCD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

GTX100209 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Atif SM et al. JCI Insight 2019; 5 Protective role of B cells in sterile particulate-induced lung injury.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169