CD20 antibody [C2C3], C-term

Name: CD20 antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX100209
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-CD20 antibody [C2C3], C-term used in Western Blot (WB). GTX100209

Anti-CD20 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100209

Anti-CD20 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100209

Cat. No. GTX100209

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all CD20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

K562 , THP-1

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD20. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1080937

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7

Cellular Localization

Membrane

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]

Database

Gene ID: 931 MS4A1
UniProt: P11836 MS4A1

Research Area

DATA IMAGES

GTX100209 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted withCD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100209 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100209 ICC/IF Image

CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD20 protein stained by CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100209 IHC-P Image

CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD20 stained by CD20 antibody [C2C3], C-term (GTX100209) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100209 WB Image

Sample (30 ug of whole cell lysate)
A: K562
B: THP-1
12% SDS PAGE
GTX100209 diluted at 1:10000

REFERENCE

Application Reference

Atif SM et al. JCI Insight 2019; 5 Protective role of B cells in sterile particulate-induced lung injury.

Application : IHC-P

Reactivity : Human

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