FACS: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
The antibody LT20 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Phosphate-buffered saline, pH 7.4, containing 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Normal human lymphocytes from lymph node
Purified by ion exchange chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Membrane Spanning 4-Domains A1,B1,Bp35,Cd20,Cvid5,Leu-16,Ms4A2,S7,Ms4A1
CD20 is found on all human B cells and no other cell types. CD20 is present on 9% normal peripheral blood lymphocytes.