PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 is optimal but pH 7.8 results in only slightly weaker stainings. At pH 6.0, the immunostaining is markedly reduced.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48 and BOND RX - Leica. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Positive Control
Tonsil or appendix: A strong, predominantly membranous staining of the mantle zone B-cells, the germinal centre B- cells and the interfollicular B-cells is required, while all other cells should stain negative.
Negative Control
Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any staining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length MS4A1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7
Cellular Localization
Cell membrane
Background
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/templates/default/js/lightbox/images/loading.gif "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")
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GTX04392 IHC-P Image
IHC-P analysis of human thymus tissue using GTX04392 CD20 antibody [MSVA-020R] HistoMAX™.
A relatively low fraction of CD20 positive B-lymphocytes.
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GTX04392 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04392 CD20 antibody [MSVA-020R] HistoMAX™.
In the tonsil, many lymphocytes stain positive while the surface epithelium is CD20 negative.
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GTX04392 IHC-P Image
IHC-P analysis of human mucosa from appendix tissue using GTX04392 CD20 antibody [MSVA-020R] HistoMAX™.
Strong CD20 staining of a germinal centre in the appendix mucosa while epithelial cells are completely negative.
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REFERENCE
There are currently no references for CD20 antibody [MSVA-020R] HistoMAX™ (GTX04392). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD20 antibody [MSVA-020R] HistoMAX™ (GTX04392). Be the first to share your experience with this product.
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/upload/website/prouct_img/small/GTX04392/GTX04392_20230728_IHC-P_146_23072722_155.webp "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/upload/website/prouct_img/small/GTX04392/GTX04392_20230728_IHC-P_276_23072723_330.webp "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/upload/website/prouct_img/small/GTX04392/GTX04392_20230728_IHC-P_16_23072722_969.webp "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/upload/website/prouct_img/normal/GTX04392/GTX04392_20230728_IHC-P_146_23072722_155.webp "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/upload/website/prouct_img/normal/GTX04392/GTX04392_20230728_IHC-P_276_23072723_330.webp "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")
![Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392](/upload/website/prouct_img/normal/GTX04392/GTX04392_20230728_IHC-P_16_23072722_969.webp "Anti-CD20 antibody [MSVA-020R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04392")