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CD20 antibody [SPM618]

Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475
Anti-CD20 antibody [SPM618] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34475
Anti-CD20 antibody [SPM618] used in IHC (Paraffin sections) (IHC-P). GTX34475

Cat. No. GTX34475

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM618

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all CD20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MS4A1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B1 , Bp35 , CD20 , CVID5 , LEU16 , MS4A1 , MS4A2 , S7 , membrane spanning 4domains A1 , membrane spanning 4-domains A1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475

GTX34475 FACS Image

FACS analysis of Raji cells using GTX34475 CD20 antibody [SPM618].
Blue : Primary antibody
Red : Isotype control

Anti-CD20 antibody [SPM618] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34475

GTX34475 ICC/IF Image

ICC/IF analysis of MOLT-4 cells using GTX34475 CD20 antibody [SPM618].
Green : Primary antibody
Red : nucleus

Anti-CD20 antibody [SPM618] used in IHC (Paraffin sections) (IHC-P). GTX34475

GTX34475 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34475 CD20 antibody [SPM618].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529