APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human MS4A1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B1 , Bp35 , CD20 , CVID5 , LEU16 , MS4A1 , MS4A2 , S7 , membrane spanning 4domains A1 , membrane spanning 4-domains A1
Cellular Localization
Cell membrane
Background
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475](/templates/default/js/lightbox/images/loading.gif "Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475")
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GTX34475 FACS Image
FACS analysis of Raji cells using GTX34475 CD20 antibody [SPM618].
Blue : Primary antibody
Red : Isotype control
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GTX34475 ICC/IF Image
ICC/IF analysis of MOLT-4 cells using GTX34475 CD20 antibody [SPM618].
Green : Primary antibody
Red : nucleus
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GTX34475 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34475 CD20 antibody [SPM618].
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REFERENCE
There are currently no references for CD20 antibody [SPM618] (GTX34475). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD20 antibody [SPM618] (GTX34475). Be the first to share your experience with this product.
![Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475](/upload/website/prouct_img/small/GTX34475/GTX34475_20200115_FACS_1780_w_23060801_203.webp "Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475")
![Anti-CD20 antibody [SPM618] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34475](/upload/website/prouct_img/small/GTX34475/GTX34475_20200115_ICC-IF_1609_w_23060801_188.webp "Anti-CD20 antibody [SPM618] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34475")
![Anti-CD20 antibody [SPM618] used in IHC (Paraffin sections) (IHC-P). GTX34475](/upload/website/prouct_img/small/GTX34475/GTX34475_20200115_IHC-P_1290_w_23060801_900.webp "Anti-CD20 antibody [SPM618] used in IHC (Paraffin sections) (IHC-P). GTX34475")
![Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475](/upload/website/prouct_img/normal/GTX34475/GTX34475_20200115_FACS_1780_w_23060801_203.webp "Anti-CD20 antibody [SPM618] used in Flow cytometry (FACS). GTX34475")
![Anti-CD20 antibody [SPM618] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34475](/upload/website/prouct_img/normal/GTX34475/GTX34475_20200115_ICC-IF_1609_w_23060801_188.webp "Anti-CD20 antibody [SPM618] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34475")
![Anti-CD20 antibody [SPM618] used in IHC (Paraffin sections) (IHC-P). GTX34475](/upload/website/prouct_img/normal/GTX34475/GTX34475_20200115_IHC-P_1290_w_23060801_900.webp "Anti-CD20 antibody [SPM618] used in IHC (Paraffin sections) (IHC-P). GTX34475")